Saturday, January 8, 2011
... purpose
... i've photographed them before but there was a fortuitous sunbeam this morning as we waited to cram in to the final weekend of the monumental diaghilev exhibition
Friday, January 7, 2011
how others see things ... easy when you know how
Sub-confluent, interphase Human Umbilical Vein Endothelial Cells (HUVECs) transfected with GFP-WT-MCAK (Mitotic Centromere Associated Kinesin) and grown in culture for 8 hours.
Cells were fixed in PHEM buffer containing 4% paraformaldehyde, 0.05% gluteraldehyde, and 0.2% Triton X-100. Cells were then labeled with mouse anti-tubulin detected by goat anti-mouse Alexa 568 antibody.
Cells were imaged on a spinning disk confocal microscope using a 60x 1.4 NA oil immersion objective lens on a Nikon Eclipse Ti equipped with Perfect Focus System and a Yokogawa CSU-X spinning disk confocal scan head equipped with a multi-bandpass dichromatic mirror (Semrock; Rochester, NY) and bandpass filters (Chroma; Rockingham, VT) in an electronic filterwheel for selection of GFP or Texas red emission.
Excitation light provided by a custom-built laser combiner module (modification of LMM-3, Spectral Applied Research; Richmond Hill, Ontario, Canada) containing 500mW solid state lasers (488 nm: Coherent; 561 nm: MPB Communications; Montreal, Quebec) that were shuttered with electronic shutters and attenuated and/or directed to a fiber-coupled output port with an AOTF (Neos Technologies, Melbourne, FL) and directed to the confocal scan-head via a single-mode optical fiber (Oz Optics, Ottawa, Ontario, Canada).
A Coolsnap HQ2 camera captured images with 200ms exposure @ 488nm and 400ms exposure @ 560nm.
http://cellimagelibrary.org/home
designing her personalized karmic index
the news on radio and television, good or bad, is almost always followed with a brief summary of movements in the main financial markets around the world
perhaps those not even moderately wealthy in spiritual and intellectual capital needs must measure our human condition in similar ways
therefore, in an altruistic surge of activity destined to improve the happiness of the entire human race, i am prototyping and developing the loved one's karmic index
of course, in the life of an almost perfect woman, karmic index movements are generally upwards, but uncharacteristic glitches may occur
for instance, when i returned to the master bedroom after an unplanned visit to the loo before falling asleep last night, i was deeply disappointed to find that she had carried on reading her terry pratchett and had not even thought of plumping my pillows
i shall be posting frequent re-assessments using a simple logarithmic scale with the daily to-do lists that i like to leave around the flat for her
it is for her own good
perhaps those not even moderately wealthy in spiritual and intellectual capital needs must measure our human condition in similar ways
therefore, in an altruistic surge of activity destined to improve the happiness of the entire human race, i am prototyping and developing the loved one's karmic index
of course, in the life of an almost perfect woman, karmic index movements are generally upwards, but uncharacteristic glitches may occur
for instance, when i returned to the master bedroom after an unplanned visit to the loo before falling asleep last night, i was deeply disappointed to find that she had carried on reading her terry pratchett and had not even thought of plumping my pillows
i shall be posting frequent re-assessments using a simple logarithmic scale with the daily to-do lists that i like to leave around the flat for her
it is for her own good
Thursday, January 6, 2011
Wednesday, January 5, 2011
Monday, January 3, 2011
fluid mechanics ... the armitage shanks mystery
at the national gallery there's an astonishing exhibition of paintings by "canaletto and his rivals"
and there's a smaller exhibition of some paintings by bridget riley
each shows the power of the human imagination synergized by the precision of the artist's perceptions and dexterity
then some of us can go to the gents where precision and dexterity seem to be unimaginably difficult for the males of the species among the museum-going public and the floors are rarther slippery
Sunday, January 2, 2011
history ... out with the old ! ...
although it took me a month to get to page 573,
i wish i could have read this wonderful book in 1984 when it was first published
and it is a mystery why the labour government never got around to honouring the lady
... even though there is no mention in the text of the saltonstall wives depicted on the cover
http://en.wikipedia.org/wiki/Antonia_Fraser
Saturday, January 1, 2011
intolerable cruelty
Floating upwards from a night of dreams into voluptuous consciousness, just before dawn certain localized sharp movements and non-verbal vocalizations alert me to the loved one’s wakefulness, and so I venture to speak.
“I dreamed I woke up on my day off and someone made me a cup of tea.”
She whose given middle name is Sardonique, replies.
“That was only a dream.”
Chinese winter sports ... a mystery wrapped in a puzzle wrapped inside an enigma ...
Clearly, he thinks he's hard, but look at those nancy-boy gloves, and he's only playing against a bunch of women.
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